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. 2005 Jun;25(12):5183–5195. doi: 10.1128/MCB.25.12.5183-5195.2005

FIG. 1.

FIG. 1.

In vitro growth of parental and transduced strains of bone marrow stromal cells. (A) The population doublings of UE6E7-16, UE7T-13, UE6E7T-12, UBE6T-7, UBT-5, and H4-1 cells are shown. UE7T-13, UE6E7T-12, UBE6T-7, and UBT-5 cells proliferated for over 60 PDs and for more than 400 days. By contrast, parental H4-1 cells and UE6E7-16 cells stopped growing and entered senescence or the growth arrest stage, which is indicated by crosses. (B) H4-1 and UE6E7-16 cells entered senescence at 44 PDs and 70 PDs, respectively, approximately 200 days after the start of culture. (C) Phase-contrast photomicrographs of the bone marrow stromal cells at the semiconfluent stage in a 40-PD culture. (a) H4-1 cells; (b) UBT-5 cells; (c) UBE6T-7 cells; (d) UE6E7T-12 cells; (e) UE7T-13 cells; (f) UE6E7-16 cells.