FIG. 4.

Karyotypic analysis of parental and transduced cells with extended life spans. (A, B, C, and D) G-banded karyotyping. (E, F, and G) Spectral karyotyping. Metaphase spreads with structural chromosomal abnormalities were determined after the indicated number of PDs. Normal diploidy was seen in representative parental cells (A: H4-1) and UE7T-13 cells (B and G), but abnormalities were seen in UE6E7T-12 (C and F), UBE6T-7 (E), and UE7T-13 cells (D). Deletion and translocation are indicated by arrowheads and arrows, respectively.