FIG. 7.

RT-PCR analysis of expression of neuron-associated genes confirmed the gene chip data. RT-PCR analyses of RNAs from undifferentiated UE7T-13 cells, neuronally differentiated UE7T-13 cells, and human total brain were performed with primers that react with the human genes encoding MAP-2, tubulin 3, NF-M, Notch-1, NCAM, Sox-2, Nestin, and Nurr1. The signals of the 18S gene are approximately the same, indicating an equivalent input for all samples. Most of the neuron-associated genes were expressed by neuronally differentiated UE7T-13 cells and total brain cells.