FIG. 8.

Calcium imaging of neuronally differentiated stromal cells. (A) Phase-contrast photomicrograph of neuronally differentiated UE7T-13 cells. (B) The cell indicated by the asterisk in panel A showed rapid and reversible calcium uptake in response to high-potassium stimulation (red line; 115%), but simply changing the extracellular solution to the normal Tyrode's solution did not result in any calcium uptake (control; black line). Images obtained at 10 seconds after the high-potassium stimulation (D) and in the control (C). Rapid and reversible calcium uptake in response to high-potassium stimulation was observed in the four cells within this field.