FIG. 2.

tbTRF is an integral component of T. brucei telomeres. (A) IF combined with FISH indicates that tbTRF colocalizes with TTAGGG repeats. Endogenous tbTRF (green) is detected by AB 1261 (bloodstream) and AB 1260 (procyclic). TTAGGG repeats (pink) are detected by hybridization with a CCCUAA probe. DNA is stained with DAPI (4′,6′-diamidino-2-phenylindole) (blue). Two sets of images are shown for each life cycle stage. (B) The TTAGGG repeat DNA coimmunoprecipitates with tbTRF. DNA precipitated by ChIP with AB 1260 or preimmune serum, together with the input DNA sample (1%), was hybridized with either a TTAGGG-repeat probe or a 177-bp repeat probe as indicated. (C) Quantification of the ChIP results shown in panel B. Light gray bars represent precipitations with preimmune serum; dark gray bars represent precipitations with AB 1260. Probes used in different hybridizations were indicated at the bottom.