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tbTRF interacts with itself. (A) Deletion mutants of tbTRF used in two-hybrid analysis. Amino acid numbers (inclusive) are given. (B) Yeast two-hybrid analysis results showing that tbTRF interacts with itself. β-Galactosidase activity units measured by liquid assay with ONPG as the substrate are given as averages ± standard deviations calculated from multiple experiments (Materials and Methods). GAD, Gal4 activation domain.
