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. 2005 Jun;25(12):5011–5021. doi: 10.1128/MCB.25.12.5011-5021.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 5. — tbTRF is essential. (A) Growth curves for three independent bloodstream tbTRF^+/− tbTRF-RNAi clones (1, 3, and 5) under both induced (+Dox) and uninduced (−Dox) conditions. The population doublings (PD) and hours after induction (y axis) are indicated. (B) tbTRF protein level decreases after induction of tbTRF RNAi. Whole-cell protein extracts were prepared from bloodstream wild-type (WT) or tbTRF^+/− tbTRF-RNAi cells that express GFP-tbTRF from tbTRF endogenous locus. Western blotting was carried out with tbTRF antibody 1261.