FIG. 4.

Mrg15 expression in MrgX-deficient mice. (A) Mrg15 and MrgX mRNA expression in various tissues from wild-type (+/+) and MrgX^−/− (−/−) mice. Expression of mRNA was determined by Northern blotting. No MrgX transcript was present in MrgX^−/− tissues. EtBr staining of RNAs (28S and 18S) was the control for equal amounts of loading of samples. (B) MRG15 protein in various tissues from wild-type (+/+) and MrgX^−/− (−/−) mice was determined by Western blot analysis. Equal amounts of loading of wild-type and MrgX^−/− samples was confirmed using an anti-β-actin antibody. These results are representative of analyses of multiple male or female mice.