FIG. 4.
Nucleotide sequence analysis of HIV-1 genes in chimpanzee C459. (A) Sequence alignments of portions of gag and vpr genes of HIV-1LAV, HIV-1SF2, and HIV-1JC/NC, illustrating major differences. (B) PBMC from chimpanzee C459 obtained pre- and post-HIV-1NC infection (sample dates 11/19/96 and 9/9/97, respectively) were used to prepare genomic DNA for use as template in PCR experiments. Amplified DNA was cloned into the pGEM-T vector. Twenty clones obtained from each sample were analyzed by DNA sequencing. Results show that, prior to HIV-1NC infection, sequences were most similar to HIV-1LAV (the virus used to inoculate animal C459 in 1984) but that, after HIV-1NC inoculation, sequences were most similar to HIV-1SF2 and HIV-1JC/NC (26).