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. 2024 Sep 16;14:21641. doi: 10.1038/s41598-024-72542-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — Western blot immunochemical detection of αSyn. (A) DBS samples purified using SDS were obtained from five PD samples (lanes 1–5) and three ET samples (lanes 6–8) and subject to immunoblotting comparison of αSyn species using a panel of four antibodies which are indicated. 5 µg of sample are loaded in all lanes for all samples. Antibody SNL-4 specific for residues 2–12 of αSyn demonstrates αSyn detection at ~ 17 kDa for all samples, antibody 3H11 specific for residues 43–63 of αSyn demonstrates αSyn along with additional heavier bands that may be oligomers. Antibody EP1536Y specific for pSer129 αSyn detects this epitope at ~ 17 kDa in all samples, and antibody 5C1 specific for truncated αSyn at 125 displays detection of ~ 14 kDa band preferentially in the PD samples. Band size in kDa is shown. (B) Densitometric analysis of band detection in PD (n = 5) and ET (n = 3) samples with each of the four antibodies utilized; comparison using T-test and associated P value displayed. 5C1 has significantly enhanced detection of truncated αSyn at 125 in PD samples compared with ET. Western blot images are displayed with increased brightness and contrast for ease of viewing, with originals in Supplementary information.