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. 2005 Jun;25(12):5226–5241. doi: 10.1128/MCB.25.12.5226-5241.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 6. — Effects of membrane-targeted PAK on the negative guidance response. Neurite outgrowth in cells transfected with αPAK-CAAX (A and B) or αPAK-CAAX KD (C and D). Stained with phalloidin (A and C) or anti-HA (B and D). Neurites of cells transfected with αPAK-CAAX (E) or with αPAK-CAAX KD (F) crossing a laminin/CSPG boundary. HA-tagged cells are shown. (G) Neurites of cells transfected with either PAK construct have an increased ability to cross from laminin onto CSPG. Results are for three independent experiments (n = 6 for controls, n = 8 for αPAK-CAAX-transfected cells, and n = 14 for αPAK-CAAX KD-transfected cells).