Skip to main content
. 2024 Sep 12;15:7989. doi: 10.1038/s41467-024-52411-5

Fig. 1. Construction and characterization of synthetic condensate formed by SIDP1-GFP in vitro.

Fig. 1

a Expected interactions promoting LLPS of 8-mer peptide FW1 into synthetic condensate, and the representative interactions are presented on the line below. b The SIDP1-GFP and SIDP1 protein at protein concentration of 50 μM formed condensates in vitro (150 mM NaCl, pH = 7.4, 25 oC, without PEG2000). GFP was used as a negative control. c Light microscopy images showing two individual condensates tend to fuse together. d Fluorescence recovery of a partially photobleached droplet (n = 3). The inset shows representative images of photobleaching on the droplet. e A regime diagram illustrates the formation of SIDP1-GFP condensates at different protein and salt concentrations. Scale bars = 10 μm. Data are presented as mean ± s.d. of three biologically independent replicates. Source data provided as a Source Data file.