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. 2024 Sep 12;15:7989. doi: 10.1038/s41467-024-52411-5

Fig. 5. Controlling 2′-fucosyllactose biosynthesis by co-localization of targeted cargo enzymes.

Fig. 5

a Illustration of the synthetic condensate platform of de novo biosynthesis of 2′-fucosyllactose (2′-FL) in B. subtilis. futC, α1,2-fucosyltransferase; manB, phosphomannomutase; manC, α-D-mannose 1-phosphate guanylyltransferase; gmd, GDP-mannose 6-dehydrogenase; wcaG, GDP-L-fucose synthase. b High-performance liquid chromatography (HPLC) quantification of 2′-FL produced by different recombinant strain with SIDP1 condensate co-localizing diverse pathway enzymes. The “O” signifies that the enzyme is colocalized within the cellular condensate, while “X” represents enzymes that are not recruited. c Fluorescence microscopy images of SIDP2-GFP, SIDP4-GFP, FUSN-GFP, and 3RGG-GFP driven condensates within B. subtilis. d Effects of condensates with virous physical properties on 2′-FL production. Additionally, trends of 2′-FL concentration of the all recombinant strains are shown. Data are presented as mean ± s.d. of three biologically independent replicates. Significance (P value) was evaluated by two-sided t-test. *: p < 0.05, **: p < 0.01. Scale bars = 5 μm. Source data provided as a Source Data file.