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NSP4 prevents development of a tight polarized epithelium. MDCK-1 cells were cocultured with 50 μl containing 1 nmol of NSP4 (20 μM) or VP6 from the first day of seeding the cells onto filters. Electrical resistance measurements started on day 2 postseeding of cells on filters. Values of TER presented are means ± 1 standard deviation (n = 3). MDCK, cultivation without protein in the medium.
