TABLE 1.
Oligonucleotide primers and sizes of PCR products for competitive RT-PCR
| Gene product | Primera | Size of PCR products (bp)
|
Restriction enzyme(s) for construction of competitor DNA | |
|---|---|---|---|---|
| Target | Competitor | |||
| Insulin 1 | 5′-CCAGCTATAATCAGAGACCA-3′ | 377 | 328 | SmaI-BsgI |
| 5′-GGGCCTTAGTTGCAGTAGTT-3′ | ||||
| Insulin 2 | 5′-AGGAAGCCTATCTTCCAGGT-3′ | 401 | 293 | EcoNI-SmaI |
| 5′-ATTCATTGCAGAGGGGTAGG-3′ | ||||
| Glucagon | 5′-CTGGACAATCTTGCCACCAGGGAC-3′ | 413 | 358 | SphI-BstXI |
| 5′-CCACTACGGTTACCAGGTGGTCATGTC-3′ | ||||
| Pdx1 | 5′-TCGCTGGGATCACTGGAGCA-3′ | 365 | 247 | BlpI-MluI |
| 5′-CGGGAGATGTATTTGTTAAATAAGAATTC-3′ | ||||
| Beta2 | 5′-CTCCAGGGTTATGAGATCGTCAC-3′ | 525 | 329 | PpuMI |
| 5′-GATCTCTGACAGAGCCCA-3′ | ||||
| Glut-2 | 5′-TGGGATGAAGAGGAGACTGAA-3′ | 652 | 442 | XcmI-BsrGI |
| 5′-CATCCGTGAAGAGCTGGATCA-3′ | ||||
| Glucokinase | 5′-GATGTATTCCATCCCCGAGGACG-3′ | 410 | 291 | StyI-MscI |
| 5′-GCTCCACATTCTGCATCTCCTCC-3′ | ||||
| HPRT | 5′-GGCTTCCTCCTCAGACCGCTTT-3′ | 702 | 523 | BclI-PpuMI |
| 5′-AGGCTTTGTATTTGGCTTTTCC-3′ | ||||
a
The sequence of the forward primer is given on the top, and that of the reverse primer is given on the bottom.