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Detection of calnexin levels by Western blotting after transient transfection. CHO cells stably expressing either wt CFTR (A) or F508del-CFTR (B) were transiently transfected with human calnexin cDNA (see Materials and Methods). Lanes 1, nontransfected cells; lanes 2, mock-transfected cells (with the empty vector); lanes 3, cells analyzed 24 h posttransfection with calnexin cDNA. Blots were probed with an Ab specific for human calnexin, as indicated on the left.
