FIG. 4.

SUMO-modified LRH-1 is localized in PML-containing nuclear bodies. (A) Untransfected and SUMO-siRNA-transfected C3A-HepG2 cells were stained with αLRH-1 (rows 1 and 3) and αSUMO-1 (rows 2 and 4). (B and C) HeLa cells, which were transfected with the expression vectors indicated at the right, were observed by direct fluorescence of YFP or immunofluorescence staining with anti-Flag or anti-RNA Pol II antibodies (left panels). In all cases PML-NBs were detected in the same field by immunofluorescence staining with a TRITC (tetramethyl rhodamine isothiocyanate)-conjugated PML antibody (middle panels). Merged images are depicted in the right panels.