FIG. 3.

Binding kinetics of SAGA, Mot1p, Taf1p, TBP, and Pol II to HXT genes in SAGA and MOT1 mutant strains after a shift to low glucose. (A) Representative PCR and PhosphorImager quantification of Mot1p binding to HXT genes in wild-type (Wt), gcn5Δ, spt3Δ, and spt8Δ cells. The top panel shows multiplex PCR analysis in which POL1 primers have been included as a normalization control. Primers amplifying HXT2 and HXT4 are specific for the core promoter region as indicated for Fig. 2A. Each sample was analyzed at least in duplo. The lower panel displays the quantification. Factor binding is expressed as HXT/POL1 ratios, as described previously (1). (B) Analysis of Spt20p binding to HXT genes in a mot1-1 mutant strain after a shift to low glucose. (C) Taf1p binding to HXT in Wt, gcn5Δ, spt3Δ, and spt8Δ cells. (D) TBP and Pol II recruitment to HXT genes in Wt, gcn5Δ, spt3Δ, and spt8Δ strains.