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Binding kinetics of Mot1p, Taf1p, TBP, and Pol II to HXT genes in SAGA double deletion strains. (A) Recruitment of Mot1p, Taf1p, TBP, and Pol II in gcn5Δ spt3Δ and gcn5Δ spt8Δ cells after a shift to low glucose. Multiplex PCR signals of immunoprecipitated DNA as indicated above the lanes are displayed for the TATA-containing fragments of HXT2 and HXT4 as described for Fig. 2A. (B) PhosphorImager quantification of Mot1p, Taf1p, TBP, and Pol II binding to the indicated HXT promoters.
