FIG. 4.

Changes in histone H3 and H4 acetylation are triggered by homologous recombination. A. Cutting and repair of the HO site were measured when the HO endonuclease was induced at 0 h and repressed at 2 h in strain JKM179 (hml/hmrΔ) containing plasmid pGAL-HO-URA3. B. An H3 C-terminal (H3C-term) antibody was used to measure the relative levels of histone proteins 0.6 kb away from the HO site by ChIP, real-time PCR, and statistical analyses as described in the legend to Fig. 2. C. Levels of histone acetylation in strain JKM179 (hml/hmrΔ) containing plasmid pGAL-HO-URA3 at 0.6 kb from the HO site were measured as described for panel B and in the legend to Fig. 2. Significant changes are noted by small light grey circles as described in the legend to Fig. 2.