FIG. 2.

Expression of Axin2 and Axin in Axin^AX2 and Axin^AX mice. (A) Protein lysates from wild-type (WT) and Axin^Ax/+ heterozygous embryos were probed with anti-Axin antibodies. The heterozygotes expressed equal amounts of Myc-tagged Axin from the Axin^AX allele and wild-type Axin from the endogenous allele, showing that the Axin^AX allele is expressed at a normal level. (B, C, and D) Protein lysates from wild-type, Axin^AX/AX, and Axin^AX2/AX2 homozygous embryos were probed with anti-Myc antibody (B) or with two different anti-Axin antisera (C and D). In wild-type embryos, the anti-Axin antibodies detected endogenous Axin (∼110 kDa), while the anti-Myc antibody detected only background bands. In Axin^AX/AX embryos, both the anti-Axin and the anti-Myc antibodies detected Myc-tagged Axin (∼130 kDa), while endogenous Axin was absent. In Axin^AX2/AX2 embryos, the anti-Axin antibodies detected only background bands also seen in wild-type embryos, while the anti-Myc antibody detected Myc-tagged Axin2. Anti-Axin antiserum FF, a gift from François Fagotto, was raised against amino acids 406 to 685 of mouse Axin, while anti-Axin antiserum DV, a gift from David Virshup, was raised against full-length mouse Axin. Molecular size markers (in kilodaltons) are noted at the left of blots.