FIG. 4.

TAF9b interacts with the same partners, as does TAF9, in vitro. (A) TAF9 or (B) TAF9b was coexpressed with the indicated TAFs (TAF5 and TAF6) in Sf9 cells using the baculovirus expression system. From these cells, protein extracts were prepared, and either TAF9 (A) or TAF9b (B) was immunoprecipitated (IP) with the corresponding antibodies. From the same extracts, a control IP was performed with an unrelated antibody. Resin-bound proteins were then washed, boiled, separated by SDS-PAGE, and analyzed by Western blot using the indicated antibodies. (C) Summary of the interaction study carried out using the baculovirus expression system between either TAF9 or TAF9b and the indicated TFIID subunits.