FIG. 7.

(A) Effect of Gadd45a on EF-1α-mediated microtubule bundling. Microtubule assembly and/or bundle assays were carried out by using rhodamine (or fluorescein)-labeled tubulin according to the manufacturer's manual. The assays were performed in the presence of 100 ng of GST-EF-1α, GST-Gadd45a, or GST-p21 proteins and evaluated by using fluorescence microscopy. (B) Effect of Gadd45a expression on cytoskeleton. GFP-Gadd45a and GFP(Δ65-80)Gadd45a expression vectors were transfected into HeLa cells. After 36 h, cells were stained with antibody to β-tubulin and detected by fluorescence microscopy for green fluorescent protein (green fluorescence) or rhodamine staining (red fluorescence).