FIG. 8.
Interaction of Gadd45a with EF-1α contributes to Gadd45a-induced apoptosis and growth suppression. (A) HeLa cells were cotransfected with pCS2MT (Myc-tagged empty vector) or the indicated Myc-tagged expression vectors for Gadd45a, (Δ60-85)Gadd45a, EF-1α, or p21waf1/cip1. After selection with G418 for 2 weeks, cells were fixed, and the colonies that contained at least 50 cells were counted. Quantitative results represent the average of three individual experiments. (B) HeLa cells were transiently transfected with Myc-tagged Gadd45a or Myc-tagged (Δ60-85)Gadd45a. (C) At 48 h posttransfection, cells were harvested for the assay of DNA fragmentation and for analysis of Bim induction in mitochondria.