Fig. 3.

Rescue of defective non-canonical NF-κB signaling in P1 fibroblasts. (A) SV40-F from a healthy control (C) and P1 were stably transduced with an empty vector (EV) or the WT RELB cDNA. SV40-F from the healthy control and P1 were also transduced with the Q72Tfs, E145K, P364L, or Y397* mutant RELB cDNA. The RNA extracted from these cells was subjected to RT-qPCR for total RELB. Data are displayed as 2^−ΔCt relative to EV-transduced cells, after normalization against GUSB (endogenous control) expression (ΔCt). The result of one representative experiment is shown. (B) SV40-F from a healthy control (Left) or from P1 (Right) were stably transduced with an EV, the WT or a mutant (Q72fs, E145K, P364L, or Y397*) RELB cDNA. The cells were either left unstimulated (NS, gray bars for the control or red bars for P1) or were stimulated for 24 h with Lt (black bars). The RNA extracted from these cells was subjected to RT-qPCR for total NFKB2. Data are displayed as 2^−ΔCt values relative to unstimulated EV-transduced cells, after normalization against GUSB (endogenous control) expression (ΔCt). The result of one independent experiment is shown.