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FLIP upregulates Bcl-XL in MLEC. MLEC at 30% confluence were cultured in serum-free media in the presence of 106 CPU/ml of adeno-FLIP or adeno-lacZ for 3 h and then restored to normal medium. Two days later, cells were exposed to hypoxia (95% N2, 5% CO2) for 24 h and then restored to normal culture conditions (95% air, 5% CO2). At the indicated reoxygenation times, cell lysates were subjected to Western blot analysis to detect Bcl-XL.
