TABLE 2.

Oligonucleotides used in this study^a

Name	Description	Sequence
OBL163	TRM9 deletion 5′	ATTCGGTAATGAACTGAACAGAGATGAGGTCTCGAAGAGCCAAGAAATAACGGGTGTTGGCGGGTGTC
OBL164	TRM9 deletion 3′	CCTGCTGCTACAAAATACACTGTCTACCTATATATCACCTTCATCTCTTCCTGATGCGGTATTTTCTCCT
OBL185	MTC6 5′	TTCAGCTAAAAGTTGTTCG
OBL186	MTC6 3′	TGATTTATATGGTTGTGGTG
OBL197	TRM11 5′	TACCCGTAATATGGAGATG
OBL198	TRM11 3′	TTTTTCACTTGTATTTTTG
OBL230	TRM1 deletion 5′	TCGCAAAGTTACAGATCCTGAGCAGTCATAAGTTGATACCTTTCCTCTTACACGGGTGTTGGCGGGTGTC
OBL231	TRM1 deletion 3′	ATATATATTTATTTATTTTTTACACATACATACTGCCCTCCTGATTACTTCTGATGCGGTATTTTCTCCT
OBL236	TRM11 cloning 5′	AACTGCAGATTACAGTGGCGGAAAGGAG
OBL237	TRM11 cloning 3′	CCCAAGCTTAATGCGGAGCTGATAAGG
OBL238	TRM11-TAP 5′	TAAAACGTTCAAAAGATAATTTCAGGGAGCGGTACTTCAATAATTTTAACTCCATGGAAAAGAGAAG
OBL239	TRM11-TAP 3′	TTCTACATGATGGAACATAAACATAAATATAGGTATAGATAAATTGTTCTTACGACTCACTATAGGG
OBL244	TRM11-D215A 5′	GAACTATAATGTACGCCCCGTTTGCAGGTAC
OBL245	TRM11-D215A 3′	GTACCTGCAAACGGGGCGTACATTATAGTTC
OBL246	TRM11-D291A 5′	GATACTATTTTGTGTGCTCCTCCATATGGTA
OBL247	TRM11-D291A 3′	TACCATATGGAGGAGCACACAAAATAGTATC
OBL250	LYS9 deletion 5′	GTAGGGAAAACTTATCAGGACAATTGAGTTATATTAACGTATTATATATT CGGGTGTTGGCGGGTGTC
OBL251	LYS9 deletion 3′	AATAGCCAATACTTTTTAGACTAAAGGTAGACGACTTACTAGCGGTTCAACTGATGCGGTATTTTCTCCT
OBL254	TRM112 5′	AATCAGCATCCTCAAACT
OBL255	TRM112 3′	GTTGAAGGGAATCGTGTT
OBL282	TRM112-TAP 3′	ACATGCATGCGTTGAAGGGAATCGTGTT

^aNucleotides in boldface type correspond to the mutations that were introduced in TRM11.