FIG. 7.

p300 is recruited to the Grp78 promoter in Tg-stressed cells, and its enhancing activity is dependent on YY1. (A) The ChIP assays were performed with HeLa cells as described in the legend to Fig. 6B. The immunoprecipitation assays were carried out with normal IgG or antibodies against p300, acetylated histone H4, and histone H3, and the primers for PCR are identical to the ones used in the experiment shown in Fig. 6B. (B) CV-1 cells were transfected and assayed for luciferase activity as described in the legend to Fig. 6E, except 0.1 μg of p300 expression plasmid was cotransfected as indicated.