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The NRS-Ad3′ RNA generates a stable presplicing complex. Wild-type NRS, UU/AA, and UU/CC NRS-Ad3′ sequences were incubated in a splicing reaction for 0, 8, or 25 min at 30°C, heparin was added, and reactions were electrophoresed through a nondenaturing agarose gel. Positions of H, A, and B/C splicing complexes are indicated on the left.
