Skip to main content
. Author manuscript; available in PMC: 2024 Sep 17.
Published in final edited form as: Nat Protoc. 2023 Nov 23;19(2):487–516. doi: 10.1038/s41596-023-00914-8

Table 2 ∣.

Examples of media and coatings for single-cell isolation with CellRaft

Cell type Culture mediuma Manufacturer,
cat. no.		 Coating Recommended
concentration		 Manufacturer,
cat. no.
Fibroblast FGM Lonza, CC4126 n.a n.a n.a
Hepatocyte Hepatocyte plating medium Lonza, MP-100 Collagen type I 10 μg/mL in PBS Sigma, C3867
Lymphocyte RPMI 1640 Gibco, 11875093 Gelatin type B 2% solution in H2O Sigma, G1393
Nuclei (e.g., neuronal) PBS + 5% FBS See reagent list Poly-l-lysine 0.01% solution in H2O Sigma, P4707
a

We list the basic medium here. Different supplements may be used depending on culture purposes or conditions.