| Reagent | Volume per sample (μL) |
|---|---|
| Fast SYBR Green master mix | 5 |
| Locus-specific primers (1 μM) | 1 |
| Nuclease-free water | 2 |
| SCMDA sample (1 ng/μL) or genomic DNA (1 ng/μL) or H2O | 2 |
| Total volume | 10 |
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