Figure 7.

BRF1A enhanced the mRNA and protein expression of telomerase reverse transcriptase in IgE-producing myeloma cells. Myeloma cells were cultured with BRF1A at 1 µM for 48 hours, and mRNA was determined using qRT-PCR as described in the section “Materials and Methods.” mRNA expression of (A) telomere. (B) hTERT. Western blotting assay was conducted as described in the section “Materials and Methods.” Protein expression of (C) TERT. (D) p-TERT. (E) Western bands.

Data represent triplicate experiments and are expressed as mean ± SD. *P < .05; **P < .01; ***P < .001 versus control (0.1% DMSO).