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Sedimentation velocity centrifugation. Wild-type and mutant T antigens were incubated in the presence or absence of Hsc70 plus 4 mM MgATP and then layered onto a 5 to 15% glycerol gradient. The reaction mixtures were centrifuged, and fractions were collected. (A) Fractions were analyzed via SDS-PAGE and Coomassie blue staining. (B) The relative percent of the total Hsc70 or T antigen in each fraction was graphed.
