TABLE 1.
Mutant T antigen association with Hsc70a
| T antigen protein | Binds Hsc70 + ATP | Binds Hsc70 − ATP | Binds Hsc70(1–371) + ATP |
|---|---|---|---|
| TAg | + | − | − |
| D44N | − | − | NDb |
| D15K | + | − | ND |
| 5061 | + | − | ND |
| N136 | − | − | − |
| N136, D44N | − | − | ND |
| N136, K1 | − | − | ND |
a
Purified T antigen or mutants of T antigen were incubated in the presence (+) or absence (−) of MgATP with Hsc70 or the ATPase fragment of Hsc70 (Hsc70(1–371)). These reactions were then immunoprecipitated with antibodies to T antigen, and the ability to coimmunoprecipitate Hsc70 was determined.
b
ND, not determined.