TABLE 3.
Nucleotide and deduced amino acid changes in human sapovirus genomes during scale-up passages
| Sapovirus genotype | Strain | Accession number | Sequenced samples | Nonstructural proteins (NS) | VP1 | VP2 | Summary of amino acid changes |
|---|---|---|---|---|---|---|---|
| GI.1 | FS40 | LC788817a | P1 only | Not comparable | |||
| GI.2 | FS25 | LC788818a | P1 and P2 | NS2-K195E (AAA→GAA) P2 |
NS2 in P2 | ||
| GI.3 | D1736 | AB522396 | original, P1, and P2 | NS2-T96A (ACA→GCA) P1 NS2-A209G (GCA→GGA) P1 NS7-C1555b (TGC→TGT) P1 |
P2 domain-D2111H (GAT→CAT) P2 |
NS2 in P1 and VP1 in P2 | |
| GI.4 | Chiba000496 | AJ606693 | Original, P1, and P2 | P2 domain-S2088P (TCA→CCA) P1 T2163b (ACC→ACT) P1 P1 domain-R2277S (AGA→AGT) P1 |
VP1. P2 was identical to P1 |
||
| GI.5 | D1729 | AB522393 | Original and P1 | NS4-I792T (ATC→ACC) P1 NS4-L903b (CTC→CTT) P1 NS7-P1417b (CCC→CCT) P1 |
P1 domain-Q2286 stop (CAA-TAA) P1 |
NS4 and VP1. P2 was identical to P1 |
|
| GI.6 | Chiba000764 | AJ606694 | Original and P1 | NS7-Y1276b (TAT→TAC) P1 NS7-V1407I (GTC→ATC) P1 |
P1 domain-R2280S (AGA→AGT) P1 |
NS7 and VP1 in P1 | |
| GI.7 | D1714 | AB522390 | Original, P1, and P2 | NS2-K168E (AAG→GAG) P1 |
NS2. P2 was identical to P1 |
||
| GII.1 | Kumamoto129 | AB689845 | Original, P1, and P2 | Not changed | |||
| GII.2 | Kumamoto130 | AB689846 | Original and P1 | Not changed | |||
| GII.3 | FS130 | LC788819a | P1 only | Not comparable | |||
| GII.4 | D1739 | AB522399 | Original, P1, and P2 | Shell domain-S1923P (TCG→CCG) P2 |
VP1 in P2 | ||
| GII.5 | Kashiwa1 | LC190463 | Original, P1, and P2 | NS1-P60b (CCG→CCT) P1 |
F1923b (TTT→TTC) P2 |
Not changed | |
| GII.8 | AK764 | LC787637a | Original, P1, and P2 | Not changed | |||
| GV.1 | D3302 | LC787639 | Original and P1 | NS4-I800T (ATA→ACA) P1 |
NS4 in P1 | ||
| GV.2 | NGY1 | AB775659 | Original, P1, and P2 | NS2-G76D (GGT→GAT) P1 NS3-T558A (ACA→GCA) P2 NS4-R677C (CGT→TGT) P1 NS6-D1115N (GAC→AAC) P2 |
P1 domain-E2231D (GAG→GAT) P1 P2 domain-V2099A (GTT→GCT) P2 |
NS2,4 and VP1 in P1 and further in NS3, NS6, and VP1 in P2 |
a
Sequence from P1 virus.
b
Synonymous nucleotide changes.