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. 2024 Aug 27;9(9):e00545-24. doi: 10.1128/msystems.00545-24

Fig 1.

Study involves 100 fish reared individually, split into antibiotic and control groups, then further divided into parasite and control treatments. Samples and checks include fecal 16S, metabolome, and histopathology at different time points.

Schematic of zebrafish husbandry and treatment events and timeline. (1) Briefly, 100 adult fish were placed in individual tanks, (2b) half of the fish were subsequently exposed to antibiotics, (3b) then fish were randomly exposed to the zebrafish parasite Pseudocapillaria tomentosa. Fecal samples were collected (2a) prior to antibiotic exposure, (3a) just prior to parasite exposure, and (4) 29 days post-parasite exposure (dpe) after which fish intestinal histopathology was assessed. Samples were split and processed for untargeted fecal metabolomic analysis as well as fecal 16S rRNA DNA amplicon sequencing.