Table 1.
Strains and plasmids used in this study
| Strain | Genotype or Description | Source |
|---|---|---|
| B. cereus | ||
| ATCC14579 | Wild type | [28] |
| ΔessC (essC gene deleted mutant) | This study | |
| ΔesxA1 (esxA1 gene deleted mutant) | This study | |
| ΔesxA2 (esxA2 gene deleted mutant) | This study | |
| ΔtigR (tigR gene deleted mutant) | This study | |
| ΔesxA1ΔesxA2 (esxA1 and esxA2 genes deleted mutant) | This study | |
| ΔesxA1ΔesxA2 + pGFP78::esxA1 (ΔesxA1ΔesxA2 mutant complimented with its native esxA1 gene) | This study | |
| ΔesxA1ΔesxA2 + pGFP78::esxA2 (ΔesxA1ΔesxA2 mutant complimented with its native esxA2 gene) | This study | |
| ΔesxA1ΔesxA2 + pGFP78 (ΔesxA1ΔesxA2 mutant complimented with pGFP78 plasmid) | This study | |
| Wild type + pGFP78 (Wild type complimented with pGFP78 plasmid) | This study | |
| E. coli | ||
| SM10 | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Km) | [29] |
| S17-1 | thi, pro, hsdR, RP4-2 Tc::Mu Km::Tn7 (Tp Sm) | [29] |
| DH10β (C3019H) | Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) | New England BioLabs |
| Plasmids | ||
| pRP1028 | Bacillus and E. coli shuttle vector with turbo-rfp gene and an I-SceI recognition site; SpeR | [26] |
| pRP1099 | Vector with turbo-AmCyan and I-SceI genes; KanR | [26] |
| pGFP78 | Shuttle vector for Bacillus and E. coli containing gfp gene and F78 promoter; AmpR, TetR | [27] |
| pYF07 | pRP1028 with upstream and downstream regions of essC | This study |
| pHKK100 | pRP1028 with upstream and downstream regions of esxA1 | This study |
| pHKK102 | pRP1028 with upstream and downstream regions of esxA2 | This study |
| pHKK104 | pRP1028 with upstream and downstream regions of tigR | This study |
| pHKK114 | pGFP78 Δgfp with esxA1 | This study |
| pHKK115 | pGFP78 Δgfp with esxA2 | This study |
SpeR, KanR, TetR, and AmpR stand for spectinomycin, kanamycin, tetracycline, and ampicillin resistance, respectively