Fig. 3.
House dust mite and ozone co-exposure induce lung inflammation A) Peribronchial Inflammation B) Perivascular Inflammation. n = 5–9 mice per group and analyzed by Kruskal–Wallis test followed by Dunn's multiple comparison post hoc test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001C). Lung tissue real-time PCR analyses for mRNA expression D) BAL fluid ELISA E) Lung homogenate ELISA. n = 4–7 mice per group and analyzed by two-way analysis of variance (ANOVA) followed by Tukey's post hoc test. PCR values are presented as Log 2-fold change values. * Represents significantly different from PBS + Air while # represents significantly different between HDM + Air and HDM + O3.