KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| Wee1 (D10D2) mAb Rabbit | Cell Signaling Technology | CAT# 13084T; RRID: AB_2713924 |
| Anti-GCN2 (phospho T899) antibody | Abcam | CAT# ab75836; RRID: AB_1310260 |
| GCN2 antibody | Abcam | CAT# ab157775; RRID: |
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit | Cell Signaling Technology | CAT# 2211S; RRID: AB_331679 |
| HSP90 Rabbit mAb | Cell Signaling Technology | CAT# 4877S; RRID: AB_2233307 |
| ATF-4 (D4B8) Rabbit mAb | Cell Signaling Technology | CAT# 11815S; RRID: AB_2616025 |
| Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb | Cell Signaling Technology | CAT# 3398; RRID: AB_2096481 |
| eIF2α (D7D3) XP® Rabbit mAb | Cell Signaling Technology | CAT# 5324; RRID: AB_10692650 |
| CDK1 | Cell Signaling Technology | CAT# 28439S; RRID: AB_2798959 |
| phospho-CDK1 | Cell Signaling Technology | CAT# 9111S; RRID: AB_331460 |
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| Bacterial and virus strains | ||
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| Endura™ Chemically Competent Cells | Lucigen | Cat# 60240-1 |
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| Chemicals, peptides, and recombinant proteins | ||
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| Polyethylenimine (PEI) | Polysciences | Cat# 23966-1 |
| Polybrene | Millipore-Sigma | Cat# TR-1003-G |
| Opti-MEM | Thermo Fisher Scientific | Cat# 31985088 |
| RPMI 1640 | Corning | Cat# 15-040-CV |
| DMEM | Thermo Fisher Scientific | Cat# 11965092 |
| Bovine Growth Serum | Thermo Fisher Scientific | Cat# SH3054103HI |
| Penicillin-Streptomycin-Glutamine | Thermo Fisher Scientific | Cat# 10378016 |
| Fetal Bovine Serum | Thermo Fisher Scientific | Cat#16000044 |
| Matrigel | BD biosciences | Cat# 356237 |
| Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) | Thermo Fisher Scientific | Cat# 77720 |
| PBS antibody stabilizer | Thermo Fisher Scientific | Cat# NC0414486 |
| Paraformaldehyde (PFA) | Thermo Fisher Scientific | Cat# 28906 |
| Saponin | Millipore-Sigma | Cat# S7900-100G |
| Bovine Serum Albumin (BSA) | Millipore-Sigma | Cat# A3059 |
| Sodium azide | Millipore-Sigma | Cat# 71289 |
| Cell-ID Intercalator-Ir | Fluidigm | Cat# 201192B |
| L15 media | Millipore-Sigma | Cat# L1518 |
| Collagenase I | Millipore-Sigma | Cat# C0130 |
| Collagenase II | Millipore-Sigma | Cat# 6885 |
| Collagenase IV | Millipore-Sigma | Cat# 5138 |
| Elastase | CellSystems | Cat# LS002292 |
| DNAseI | Millipore-Sigma | Cat# 10104159 |
| RBC Lysis Buffer | Thermo Fisher Scientific | Cat# 00-4333-57 |
| AZD-1775 | SYNthesis | Cat# WZQ-0447-032 |
| Raphin1 | Tocris | Cat# 1A/242832 |
| Histogel | Thermo Fisher Scientific | Cat# NC9150318 |
| Triton X-100 | Millipore-Sigma | Cat# T8787-100ML |
| PD 407824 (Wee1 and Chk1 inhibitor) | Tocris | Cat# 2694 |
| DMSO | Sigma-Aldrich | Cat# D8418-500mL |
| RIPA buffer | Cell Signaling Technology | Cat# 9806 |
| Benzonase | Merck-Millipore | Cat# 71206-3 |
| Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23227 |
| Protein Assay Dye Reagent | Bio-Rad | Cat# 5000006 |
| Streptavidin beads | Thermo Fisher Scientific | Cat# 20357 |
| MgCl2 | Sigma | Cat# M8266 |
| ActivX™ Desthiobiotin-ATP Probe | Thermo Fisher Scientific | Cat# 88311 |
| 2x Laemmli sample buffer | Bio-Rad | Cat# 1610737 |
| PEG | Sigma | Cat# p5413 |
| QIAamp DNA Blood Maxi Kit | Qiagen | Cat# 51194 |
| BstXI | NEB | Cat# R0113S |
| BlpI | NEB | Cat# R0585S |
| QIAquick Gel Extraction Kit | Qiagen | Cat# 28704 |
| T4 ligation buffer | NEB | Cat# B0202S |
| SOC media | Sigma | Cat# S1797 |
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| Critical commercial assays | ||
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| Herculase II Fusion DNA Polymerase kit | Agilent | Cat# 600675 |
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| Deposited data | ||
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| RNA-seq data | GEO | GSE246354 |
| Raw data | Mendeley Data | https://doi.org/10.17632/f5jtt3s8hv.1 |
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| Experimental models: cell lines | ||
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| 293T | ATCC® | HTB-3216™ |
| NCI-H82 | ATCC® | HTB-175™ |
| NJH29 | Sage Lab | N/A |
| NCI-H69 | ATCC® | HTB-119™ |
| NCI-H2081 | ATCC® | CRL-5920™ |
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| Experimental models: organisms/strains | ||
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| NOD-scid IL2Rgammanull mice (NSG mice) | The Jackson Laboratory | #005557 |
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| Oligonucleotides | ||
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| Genome-Wide CRISPR-KO library | Morgens et al.,62 | N/A |
| oMCB1562: 5′-AGGCTTGGATTTCTATAAC TTCGTATAGCATACATTATAC-3′ |
Morgens et al.,62 | N/A |
| oMCB1563: 5′-ACATGCATGGCGGTAAT ACGGTTATC-3′ |
Morgens et al.,62 | N/A |
| oMCB1439: 5′-CAAGCAGAAGACGGCAT ACGAGATGCACAAAAGGAAACTCACCCT-3′ |
Morgens et al.,62 | N/A |
| R: 5′-AATGATACGGCGACCACCGAGATC TACACGATCGGAAGAGCACACGTCTGAA CTCCAGTCACNNNNNNCGACTCGGTGC CACTTTTTC-3′ |
Morgens et al.,62 | N/A |
| Sequencing primer: 5′-GCCACTTTTTCAAG TTGATAACGGACTAGCCTTATTTAAACTTG CTATGCTGTTTCCAGCTTAGCTCTTAAAC-3′ |
Morgens et al.,62 | N/A |
| EIF2AK4_2 TTGGGAGGCCATTTACGGCG GTTTAAGAGC |
This paper | N/A |
| sgRNA cloning: EIF2AK4_2_R TTAGCTCTT AAACCGCCGTAAATGGCCTCCCAACAAG |
This paper | N/A |
| sgRNA cloning: EIF2AK4_4 TTGGCCGTTCT GTGTCCTCCTGTTTAAGAGC |
This paper | N/A |
| sgRNA cloning: EIF2AK4_4_R TTAGCTCTT AAACAGGAGGACACAGAACGGCCAACAAG |
This paper | N/A |
| sgRNA cloning: PPP1R15B_4 TTGGATCGC CGAGGGAAAAAGGGGTTTAAGAGC |
This paper | N/A |
| sgRNA cloning: PPP1R15B_4_R TTAGCTCT TAAACCCCTTTTTCCCTCGGCGATCCAACAAG |
This paper | N/A |
| sgRNA cloning: PPP1R15B_8 TTGGTTACTG AGTATTATATAAGGTTTAAGAGC |
This paper | N/A |
| sgRNA cloning: PPP1R15B_8_R TTAGCTCTT AAACCTTATATAATACTCAGTAACCAACAAG |
This paper | N/A |
| sgRNA safe: GCTAGCTTGTTACTGAGCAT | This paper | N/A |
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| Recombinant DNA | ||
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| pRSV-Rev | pRSV-Rev was a gift from Didier Trono | Addgene: #12253; RRID:Addgene_12253 |
| pMDLg/pRRE | pMDLg/pRRE was a gift from Didier Trono | Addgene: #12251; RRID:Addgene_12251 |
| pCI-VSVG | pCI-VSVG was a gift from Garry Nolan | Addgene: #1733; RRID:Addgene_1733 |
| pMCB306 | pMCB306 was a gift from Michael Bassik | Addgene: #89360; RRID:Addgene_89360 |
| pMCB320 | pMCB306 was a gift from Michael Bassik | Addgene: # 89359; RRID:Addgene_89359 |
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| Software and algorithms | ||
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| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| R | R Core Team (2020) | https://www.R-project.org/. |
| CellEngine | CellCarta | https://cellcarta.com/ |
| Cytobank | Cytobank | https://cytobank.org/ |
| MEMcrispR | Drainas et al.63 | |
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| Other | ||
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| 0.22 μm filter | Millipore-Sigma | Cat# SLMP025SS |
| 70 μm strainer | Fisher Scientific | Cat# 08-771-2 |
| Superfrost Plus glass slides of 25 mm width and 75 mm length | Thermo Fisher Scientific | Cat#12-550-15 |
| Linear stainer | Leica | Cat# ST4020 |
| PT module | Thermo Fisher Scientific | Cat# A80400012 |
| CytoFLEX Flow Cytometer | Beckman Coulter | https://www.beckman.com/flow-cytometry/instruments/cytoflex |
| BD FACSAria™ III Cell Sorter | BD Biosciences | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-sorters/facsaria-iii |