Fig. 1.

CHO-K1 cells expressing VinTS alongside untransfected cells (dotted yellow boxes) were immunostained with vinculin primary antibody labeled with Alexa-647-conjugated secondary antibody in a 2D monolayer (top panels) and a 3D multicellular aggregate (bottom panels). (a, d) Alexa-647 fluorescence (red pseudocolor). (b, e) VinTS fluorescence (green pseudocolor). The white arrow in (e) indicates a region with VinTS expression but no antibody staining likely due to less uniform antibody labeling in the 3D aggregate compared with the 2D monolayers. (c, f) Merged images including Hoechst staining of the nuclei (blue pseudocolor). (g) and (h) Signal cross sections along the lines in the merged images (c and f) show overlapping peaks in the Alexa-647 and VinTS channels at sites of VinTS expression.