Table 2. Antibody source and concentration.
Antibody | Primary/ secondary | Stock concentration | Dilution | Host | Published Target Species | Source |
---|---|---|---|---|---|---|
Anti-Vinculin | Primary | 1 mg/mL | 1:200 | Mouse | Hamster | Millipore-Sigma: SAB4200729 |
Anti- Paxillin | Primary | 5.9 mg/mL | 1:250 | Mouse | Hamster | Thermo-Fisher: MA1-24952 |
Alexafluor-488 | Secondary | 2 mg/mL | 1:1000 | Goat | Mouse | Invitrogen: A28175 |
Alexafluor-647 | Secondary | 2 mg/mL | 1:1000 | Goat | Mouse | Invitrogen: A21236 |