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. 2024 Apr 1;80(4):859–871. doi: 10.1097/HEP.0000000000000865

FIGURE 2.

FIGURE 2

Immunoblot analysis of the effect of knocking down and/or knocking in the MTMR12 and FAM134A variants in the HTO/Z cell line with inducible expression of ATZ. (A) Knocking down MTMR12 with siRNA (relative densitometric values for ATZ levels compared to GAPDH levels are shown at the top and relative densitometric values for MTMR12 levels are shown above the MTMR12 blot); (B) Knocking down FAM134A with siRNA (relative densitometric values for ATZ levels compared to GAPDH levels are shown at the top and relative densitometric values for FAM134A levels are shown above the FAM134A blot); (C) ATZ, MTMR12, MTM1 and β-actin levels in HTO/Z cell line with MTMR12 variant knock-in/knockout background (relative densitometric values for ATZ levels compared to β-actin levels are shown at the top and relative densitometric values for MTM1 levels compared to β-actin levels are shown above the MTM1 blot); (D) ATZ, FAM134A and β-actin levels in HTO/Z cell line with FAM134A variant knock-in/knockout background (relative densitometric values for ATZ levels compared to β-actin are shown at the top); (E) MTM1 levels in HTO/Z cell line after removal of dox for 3 weeks, parent on the left and with MTMR12 variant knock-in/knockout background on the right (relative densitometric values on the vertical axis; n = 4 separate experiments for each bar; *p < 0.001 for students t-test). Abbreviations: ATZ, α1-antitrypsin variant Z; FAM134A, family with sequence similarity 134 member A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HTO, HeLa tet-off cell line; MTMR12, myotubularin-related protein 12; siRNA, small interfering RNA.