Fig. 5.

Restoration of hepatic Wbp2 expression rescues the impaired glucose homeostasis of Dyrk1b overexpressing mice. (a) Experimental designs following Ad-Wbp2 or Ad-EGFP injection of female C57 mice. Insulin tolerance test (b) and AUC of ITT curves (c) of Ad-EGFP (n = 6) and Ad-Wbp2 female mice (n = 7). Glucose tolerance test (d) and AUC of GTT curves (e) of Ad-EGFP and Ad-Wbp2 female mice (n = 7). Unpaired Student's t-test (two-sided) was performed for Fig. 5b–e. (f-h) Western blot and densitometry quantification of livers from Ad-EGFP and Ad-Wbp2 female mice. Two-way ANOVA with Sidak's post-hoc test was performed for Fig. 5g–h. (i) Experimental designs following Ad-Dyrk1b and Ad-Wbp2 injection of female C57 mice. Glucose tolerance test (j) and AUC of GTT curves (k) of female C57 mice injected with Ad-Dyrk1b and Ad-Wbp2 adenoviruses (n = 4–6). Insulin tolerance test (l) and AUC of ITT curves (m) of female C57 mice injected with Ad-Dyrk1b and Ad-Wbp2 adenoviruses (n = 5–6). Two-way ANOVA with Dunnett's post-hoc test was performed for Fig. 5j–m. *P < 0.05 and ***P < 0.001 for mice injected with Ad-EGFP versus Ad-EGFP and Ad-Dyrk1b, ^†P < 0.05 for mice injected with Ad-EGFP versus Ad-Dyrk1b and Ad-Wbp2, n.s., not significant. (n–o) Western blot and densitometry quantification of p-Akt in livers of female C57 mice injected with Ad-Dyrk1b and Ad-Wbp2 adenoviruses (n = 3). Two-way ANOVA with Dunnett's post-hoc test was performed for Fig. 5o. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.