TABLE 4.
Serotype Distribution of Streptococcus pneumoniae Isolated From Nasopharynx During Healthy and Acute Otitis Media Episodes, and From the Middle Ear Fluid, 2021–2023
| Healthy Nasopharynx | AOM Nasopharynx | AOM MEF | ||
|---|---|---|---|---|
| # of Isolates = 181 | # of Isolates = 79 | # of Isolates = 33 | P-values Healthy Versus AOM-NP, Healthy Versus AOM MEF | |
| PCV13 serotypes | ||||
| 19F | 6 (3%) | 2 (3%) | 2 (6%) | – |
| 3 | 5 (3%) | 1 (1%) | 1 (3%) | – |
| 23F | 1 (1%) | 0 (0%) | 0 (0%) | – |
| Total PCV13 serotypes | 12 (7%) | 3 (4%) | 3 (9%) | |
| PCV15, non-PCV13 serotypes | ||||
| 22F | 4 (2%) | 1 (1%) | 0 (0%) | – |
| PCV20, non-PCV15 serotypes | ||||
| 15B | 10 (6%) | 5 (6%) | 5 (16%) | ns, 0.06 |
| 11A | 9 (5%) | 2 (3%) | 2(6%) | – |
| Total PCV20 serotypes | 35 (20%) | 11 (14%) | 10 (31%) | |
| Non-PCV13, non-PCV15, non-PCV20 serotypes | ||||
| 35B | 29 (16%) | 27 (34%) | 7 (22%) | 0.002, ns |
| 23A | 17 (9%) | 6 (8%) | 3 (9%) | ns, ns |
| 23B | 19 (11%) | 5 (6%) | 1 (3%) | ns, ns |
| 35F | 20 (11%) | 3 (4%) | 0 (0%) | 0.06, 0.049 |
| 35D | 4 (2%) | 6 (8%) | 5 (16%) | 0.07, 0.005 |
| 21 | 8 (4%) | 3 (4%) | 2 (6%) | – |
| Nontypeable | 9 (5%) | 2 (3%) | 1 (3%) | – |
| 15C | 3 (2%) | 5 (6%) | 3 (9%) | 0.06, 0.048 |
| 16, 36 or 37 | 4 (2%) | 4 (5%) | 0 (0%) | – |
| 7 (B or C) | 5 (3%) | 1 (1%) | 0 (0%) | – |
| 28 | 5 (3%) | 1 (1%) | 0 (0%) | – |
| 6C | 4 (2%) | 1 (1%) | 0 (0%) | – |
| 15A | 4 (2%) | 1 (1%) | 0 (0%) | – |
| 10 | 4 (2%) | 0 (0%) | 0 (0%) | – |
| 13 | 2 (1%) | 1 (1%) | 0 (0%) | – |
| 24, 31 or 40 | 2 (1%) | 0 (0%) | 0 (0%) | – |
| 27, 32 or 41 | 2 (1%) | 0 (0%) | 0 (0%) | – |
| 7A | 1 (1%) | 1 (1%) | 0 (0%) | – |
| 33B | 1 (1%) | 0 (0%) | 0 (0%) | – |
| 35A | 1 (1%) | 0 (0%) | 0 (0%) | – |
| 25, 38, 43, 44, 45, 46 or 48 | 0 (0%) | 1 (1%) | 0 (0%) | – |
| Non-PCV20 serotypes | 146 (80%) | 68 (85%) | 23 (68%) | |
| Not serotyped | 2 | 0 | 1 | – |
Some strains were not fully typed (only pneumococcal-pooled sera serotyping identifications from Serum Institute was performed). Examples are group 10 and groups of serotypes such as 16, 36 or 37. Seven Spn were detected by PCR in culture-negative MEF samples that are not included in this table.