FIG. 2.

Determination of a binding site consensus from the GST-HPV₅₁E2fl CASTing. PCR amplimers from multiple rounds of the CASTing process were cloned, and the sequence of the insert was determined. Seventy-five sequences from the GST-HPV₅₁E2fl CASTing experiment were isolated, sequenced, and aligned based on homologies within the variable 20 bp of each clone. (A) The numbers represent the frequency with which a nucleotide was found at each position (expressed as a percentage of the total). Listed above the table is the consensus arrived at by determination of the most frequently occurring nucleotide at each position. (B) The frequency of specific sequences within the pool of sequenced clones expressed as a percentage of the total. Note that the first two and last two sequences listed are subsets of one another, respectively.