TABLE 3.
Locations of E2BSs in papillomavirus genomesa
| Papillomavirus | Sequence type and position
|
|||||
|---|---|---|---|---|---|---|
| LCR | LCR(ori/E) | E2 | L2 | L1 | Other | |
| HPV51 | B, 7391 | H2, 7760 | H2, 3754 | B, 4497 | H1, 5798 | |
| H3, 7742 | B, 30 | |||||
| B, 46 | ||||||
| HPV16 | B, 7457 | B, 7860 | H3, 6102 | |||
| B, 35 | B*, 6106 | |||||
| B,50 | H1, 6522 | |||||
| B*, 6565 | ||||||
| HPV11 | B, 7592 | B, 7890 | B, 4767 | B, 585/E7 | ||
| B, 35 | B, 2514/E1 | |||||
| B, 49 | ||||||
| HPV8 | B, 7401 | B, 7535 | B, 3048 | B, 4455 | H2, 6186 | |
| B, 22 | B, 3509 | H1, 5337 | B, 7367 | |||
| BPV1 | B, 7203 | B, 7896 | B, 3088 | H1, 5324 | B*, 855/E1/E7 | |
| B, 7365 | B*, 16 | B*, 2921 | B*, 1125/E1 | |||
| B, 7408 | B*, 2396/E1 | |||||
| B*, 7459 | ||||||
| B, 7510 | ||||||
| B, 7591 | ||||||
| B, 7620 | ||||||
| B, 7634 | ||||||
| B, 7760 | ||||||
Listed are all of the potential E2BSs from selected genomes based on the sequences identified previously (canonical E2BS, B = ACC-N6-GGT; B* identifies binding sites that differ from the canonical site yet were still bound by BPV1 E2 [15] or in this study (novel human E2BSs, H1 = ACAC-N5-GGT, H2 = ACC-N5-GTGT, and H3 = ACAC-N4-GTGT). The numbers correspond to the first 5 dA of the BS sequence, with nucleotide numbering corresponding to that listed in the Papillomavirus Compendium (22). These viruses represent different papillomavirus classes. HPV 51, -16, and -11 are intermediate-risk, high-risk, and low-risk mucosal types, respectively. HPV8 infects human cutaneous epithelium, and BPV infects bovine cutaneous epithelium. The regions of the papillomavirus genome where the E2BSs are found are listed at the top of the table; LCR ori/E refers to the overlapping origin of replication and early promoter in the LCR, and the other designations identify regions within the rest of the LCR or specific ORFs.