Extended Data Fig. 3. Haenkenium treatment does not affect several hallmarks of aging in muscle transcriptome and in kidneys.
a, Gene Set Enrichment Analysis (GSEA) of manually curated gene sets corresponding to different aging-related pathways in muscle tissue from HK-treated and untreated 24-month-old animals after 4 months of treatment (n = 10). b, Results of GSEA analysis shown in (a). Barplots indicate the significance of the comparison between HK-treated and untreated 24-month-old animals after 4 months of treatment. c, Western blot and densitometric analysis of of p-eIF2, p-4EBP1, p-S6, LC3A/B and PGC1 from the kidney of young (4-month-old), 24-month-old untreated, and 24-month-old treated animals after 4 months of treatment. (n = 3). Data in c are presented as mean ± SD. Statistical test used in c: One Way ANOVA with Tukey’s test. ns = not significant. Exact p values found in source data files.