Fig. 5. Aβ-driven neurodegeneration is absent in MAPT−/− iPSC-derived cortical neurons.
A Representative immunofluorescence images of Day 79–83 (Exon 1 isogenic panel) and Day 79–86 (Exon 4 isogenic panel) iPSC-derived cortical neurons treated with either 10 μM scrambled Aβ1–42 or Aβ1–42 oligomers for 5 days. The neurons were probed with antibodies against human nuclei (green) and cleaved caspase 3 (CC3; yellow) which was used as the marker for cell death. Scale bar = 100 μm. B Quantification of relative CC3+ neuron count post-treatment with either 10 μM scrambled Aβ1–42 or Aβ1–42 oligomers for 5 days. Mean ± SEM. n = three (Exon 1) or four (Exon 4) independent neuronal differentiation repeats. Two-way ANOVA with Šídák’s multiple comparison correction was used for statistical analysis compared against the scrambled Aβ1–42 treatment.