Figure 3.

GluK2 is palmitoylated at both C858 and C871 residues

Cortical neurons were incubated with lentiviruses expressing both a control shRNA and GFP (SCR) or an shRNA targeting endogenous GluK2, along with shRNA-insensitive YFP-Myc-tagged GluK2 wild type (WT) or a double cysteine mutant (C858A/C871A; C2A). Viruses were added at DIV 14, and cells were lysed 7 days later. Lysates were then subjected to the APEGS assay followed by Western blotting (WB). The GluK2 C858A/C871A mutant is not palmitoylated in neurons. Approximately 34% of GluK2 WT is palmitoylated, with ∼29% occurring at a single cysteine and ∼5% at both. “0” denotes non-palmitoylated GluK2, “1” denotes palmitoylation at one cysteine, and “2” denotes palmitoylation at two cysteines. N = 3 dissections. Error bars = ± SEM. This figure is reproduced from Yucel et al.¹