| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-myc (Rb) (used at 1:2,000) | Cell Signaling Technology | 71D10 |
| Anti-GluR6/7 (b), clone NL9 (used at 1:5,000) | Merck Millipore | 04-921 |
| Chemicals, peptides, and recombinant proteins | ||
| Poly-L-lysine solution | Merck | P4707 |
| Boric acid, 98% | Merck | B0394 |
| Sodium tetraborate (Borax) | Merck | 221732 |
| Triton X-100 | Thermo Fisher Scientific | 28313 |
| Sodium dodecyl sulfate (SDS) | Merck | L3771 |
| Bromophenol blue | Merck | B8026 |
| 2-Mercaptoethanol (βME) | Merck | 97622 |
| Tris-(2-Carboxyethyl) phosphine (TCEP) Bond-Breaker | Merck | 77720 |
| Hydroxylamine solution (NH2OH) | Merck | 438227 |
| Tetramethylethylenediamine (TEMED) | Merck | M3148-100ML |
| Methoxypolyethylene glycol maleimide (MPEG) – 10k | Merck | 99126-64-4 |
| N-ethylmaleimide (NEM) | Merck | 04256-5G |
| Ethylenediaminetetraacetic acid (EDTA) | Merck | E5134-506 |
| Chloroform, 99+% | Merck | 372978 |
| Methanol, 99.8% | Merck | 34860 |
| PageRuler prestained protein ladder, 10–180 kDa | Thermo Fisher Scientific | 26616 |
| Classico western HRP substrate, 500 mL | Immobilon | WBLUC0500 |
| Crescendo western HRP substrate, 500 mL | Immobilon | WBLUR0500 |
| Forte western HRP substrate, 500 mL | Immobilon | WBLUF0500 |
| SuperSignal West Femto maximum sensitivity substrate | Thermo Fisher Scientific | 34096 |
| Hank’s balanced salt solution (HBSS) | Gibco | 24020117 |
| Neurobasal medium (NBA) | Gibco | 21103049 |
| Trypsin-EDTA | Gibco | 25300096 |
| GlutaMAX | Gibco | 35050061 |
| Horse serum | Gibco | 26050088 |
| B27 supplement | Gibco | 17504044 |
| Penicillin-Streptomycin (5,000 U/mL) | Gibco | 15070063 |
| Complete protease inhibitor cocktail | Merck | 11697498001 |
| Phosphate-buffered saline (PBS) | Gibco | 14200-059 |
| HyClone sterile purified water | Cutiva | SH3052902 |
| Glycine | Severn Biotech | 30-21-60 |
| Stripping buffer | Thermo Fisher Scientific | 46430 |
| Ammonium persulfate (APS) | Thermo Fisher Scientific | 17874 |
| TRIS-Base | Merck | T1503-1KG |
| AcrylaGel | GeneFlow | ZT1267631S |
| Deposited data | ||
| Western blots | Yucel et al.1 | https://doi.org/10.3389/fnmol.2023.1270849 |
| Experimental models: Organisms/strains | ||
| E17 embryos of adult Han Wistar female rats | Charles River | Han Wistar |
| Recombinant DNA | ||
| pXLG3-based lentiviral construct expressing a control, non-targeting shRNA (SCR; target sequence AATTCTCCGAACGTGTCAC) and GFP | Yucel et al.1 | https://doi.org/10.3389/fnmol.2023.1270849 |
| pXLG3-based lentiviral construct expressing an shRNA targeting GluK2 (target sequence GCCGTTTATGACACTTGGA) and shRNA-resistant YFP-Myc-tagged GluK2 (wild-type or non-palmitoylatable mutant) | Yucel et al.1 | https://doi.org/10.3389/fnmol.2023.1270849 |
| Software and algorithms | ||
| Image Studio Lite | LI-COR | https://www.licor.com/bio/image-studio/ |
| Other | ||
| 6-well cell culture plate | Greiner Cellstar | GD657160 |
| Sonicator | Misonix Microson ultrasonic cell disruptor | NA |
| Hemocytometer | Improved Neubauer | NA |
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